In this paper, we present a case of meningitis caused by Toscana Virus. The patient presented to the Emergency Department with a one-day history of fever, intense back pain and headache. Physical examination revealed neck stiffness and reduced reflexes, while brain CT scan and laboratory tests were unremarkable. A lumbar puncture was performed, showing normal glucose and protein levels, and increased cell count (219 cells/µl). Bacterial antigens and CSF PCR for EBV, CMV, HIV, Herpesviruses and JCV were negative. The patient was treated with antibiotics, antivirals, steroids and mannitol. His conditions significantly improved after two days. CSF PCR finally tested positive for Toscana Virus and he was discharged with a diagnosis of meningitis due to Toscana Virus.