The authors present a case of infected non-union of the humerus treated by a two-stage procedure. In the first stage, a wide curettage of the septic tissue and a bone resection were performed introducing an antibiotic spacer. In the second stage, reconstruction of the humerus, fixation with intramedullary antibiotic nail, and bone graft were performed. A 1-year follow-up after the surgical procedure showed an excellent functional outcome, with no evidence of infection.